trypsin lysc protease mix (Thermo Fisher)

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Thermo Fisher trypsin lysc protease mix
Trypsin Lysc Protease Mix, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/trypsin+lysc+protease+mix/Trypsin/pmc12831587-312-19-22
Average 99 stars, based on 1 article reviews

trypsin lysc protease mix - by Bioz Stars, 2026-10

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Article Title: A Cyanobacterial Screening Platform for Rubisco Mutant Variants
Article Snippet: Subsequently, samples were digested by a trypsin/LysC protease mix (Pierce Trypsin/LysC protease mix, MS grade, Thermo Scientific) at a protein to protease mass ratio of 50:1 for 16 h at 37 °C with 600 rpm of shaking, after which they were quenched with formic acid to pH < 2 and centrifuged (21,000 g , 10 min, 25 °C).

Article Title: Cryo-EM structure of CLCA1 identifies CLCA1 as a founding member of a novel metzincin family
Article Snippet: The beads were then washed twice with 80% (v/v) ethanol and on-bead protein digestion was performed using Trypsin/LysC protease mix (ThermoFisher, A41007) at a 1:20 enzyme-to-protein ratio in 25 mM ammonium bicarbonate for 16 hours at 37 °C.

Article Title: Differences in photosystem II activity and carbon allocation during photomixotrophic growth in distinct wild-type strains of Synechocystis sp. PCC 6803.
Article Snippet: Samples were then alkylated with 17 mM IAA for 30 min at 25°C before being digested by a trypsin/LysC protease mix (Pierce Trypsin/LysC protease mix, MS grade, Thermo Scientific) at a protein to protease mass ratio of 50:1 for 16 h at 37 °C with 600 rpm of shaking.

Article Title: Differences in photosystem II activity and carbon allocation during photomixotrophic growth in distinct wild‐type strains of Synechocystis sp. PCC 6803
Article Snippet: Samples were then alkylated with 17 m m IAA for 30 min at 25°C before being digested by a trypsin/LysC protease mix (Pierce Trypsin/LysC protease mix, MS grade, Thermo Scientific) at a protein to protease mass ratio of 50:1 for 16 h at 37 °C with 600 rpm of shaking.

Article Title: A cyanobacterial screening platform for Rubisco mutant variants
Article Snippet: Subsequently, samples were digested by a trypsin/LysC protease mix (Pierce Trypsin/LysC protease mix, MS grade, Thermo Scientific) at a protein to protease mass ratio of 50:1 for 16 hours at 37 °C with 600 rpm of shaking, after which they were quenched with formic acid to pH < 2 and centrifuged (21,000 x g, 10 minutes, 25°C).

Article Title: A Cyanobacterial Screening Platform for Rubisco Mutant Variants.
Article Snippet: Subsequently, samples were digested by a trypsin/LysC protease mix (Pierce Trypsin/ LysC protease mix, MS grade, Thermo Scientific) at a protein to protease mass ratio of 50:1 for 16 h at 37 °C with 600 rpm of shaking, after which they were quenched with formic acid to pH < 2 and centrifuged (21,000g, 10 min, 25 °C).

Sample Prep:

Article Title: Chemical tools to define and manipulate interferon-inducible Ubl protease USP18.
Article Snippet: .. The frozen streptavidin beads were thawed at room temperature and each were added with 200μL of 1:1:1:1 100mMHEPES/lysis buffer/reducing solution/alkylating solution from the EasyPepTM MS Sample Prep Kit (Thermo, A40006) containing 3μg Trypsin/LysC protease mix (Thermo, A40007). ..

Incubation:

Article Title: Systems Biology of Recombinant 2G12 and 353/11 mAb Production in CHO-K1 Cell Lines at Phosphoproteome Level.
Article Snippet: The samples were then alkylated with 500 mM iodoacetamide (IAA) (Thermo Fisher Scientific, no. 90034, Rockford, IL, USA) for 30 min at 27 ◦C in the dark, followed by quenching with 414 μL of 50 mM TEAB. .. For in-solution digestion, 15 μL (at a 30:1 protein/protease w/w ratio) of Trypsin/LysC Protease Mix, MS Grade (Thermo Fisher Scientific, no. A40009, Rockford, IL, USA) was added and the samples were incubated overnight on a shaker at 37 ◦C. .. Finally, the digestion process was terminated with the addition of 25% trifluoroacetic acid (TFA) (Thermo Fisher Scientific, no. 28904, Rockford, IL, USA) to achieve a final concentration of 0.2% TFA in the mixture.

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